Determination Of Biochemical Changes In Amniotic Fluid According To The Number Of Offspring In Awassi Sheep.

Abstract

Fetal fluids, which are important in feto-maternal homeostasis, may be affected by the number of developing fetuses, and these effects can be evaluated by measuring some biochemical markers. Aim: The present study was carried out to investigate the difference in metabolic needs between twin and singleton pregnancies by looking at some biochemical and hormonal markers in the amniotic fluid taken at the time of birth in Awassi sheep. Materials and Methods: A total of 30 Awassi sheep were used in the study. Sheep were divided into 2 groups according to the number of offsprings by ultrasonography. The first group of the study (Group 1, n=15); ewes carrying one offspring included the second group of the study (Group 2, n=15); created sheep carrying two offsprings. From the sheep in both study groups; 10 ml of amniotic fluid was taken with a sterile syringe without disturbing the integrity of the amniotic sac hanging from the lips of the vulva at the time of delivery. Electrolyte (sodium, potassium, chlorine, calcium, phosphorus), total protein, glucose, liver (ALT and AST), kidney biomarkers (urea and keratinin) and cortisol levels were measured from the amniotic fluid. Obtained data were analyzed with independent samples t-test (Independent Sample T-test). Results: In the amniotic fluid; glucose and cortisol levels were significantly higher in the twin pregnant group than in the singleton pregnant group (p