Determination of bioactive compounds by catalytic method coupled with planar chromatography

Abstract

The promoting effect of collagen and inhibiting effect of α-alanine on the catalytic activity of copper(II) in the oxidation of 3,3′,5,5′-tetramethylbenzidine with ammonium persulfate and the reduction of iron(III) with sodium thiosulfate, respectively, have been found. A method combining planar chromatography with the subsequent catalytic determination of collagen after desorption from a plate and methods of sorption-catalytlic determination of histamine and α-alanine directly on the support have been developed with limits of detection 2 × 10−14, 5 × 10−7, and 8 × 10−14 M for histamine, α-alanine, and collagen, respectively. Separation by paper and thin-layer chromatography allowed a substantial increase in the selectivity of catalytic determination. The methods were used to determine histamine in human saliva and collagen and α-alanine in cosmetics.