Abstract
Abstract A new approach for the determination of bilirubin by an enzyme-based fiber-optic fluorescence biosensor is presented. Bilirubin is oxidized to biliverdin by bilirubin oxidase, and is quantitatively determined by measuring the fluorescence intensity change caused by the oxygen consumption in the enzymatic reaction. Bilirubin oxidase is immobilized onto an affinity membrane which is layered onto an optical indicating membrane. Two membranes are fixed around the common end of a bifurcated fiber-optic bundle. The limit of detection is 4.4 × 10−7 M. The linearity of calibration curve is between 4.4 × 10−7 M and 3.0 × 10−4 M. The reported fiber optical sensor provides a very sensitive and simple method of bilirubin measurement.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
