Abstract
Abstract A unique and practical method was developed for quantitative analysis of Bicozamycin(BCM) and its benzoylester(BCM-BZ) from yellowtail(Seriola quinqueradiata) tissues(blood, muscle, liver, kidney) by High Performace Liquid Chromatography(HPLC). Tissues were homogenized and then deprotenized with acetonitrile or a mixture of acetonitrile and water. The extract was evaporated, and the residue was dissolved in water and partitioned by addition of chloroform or carbontetrachloride. The aqueous solution was taken up into a BOND ELUT C18 cartridge column, after washing with water, BCM was eluted by methanol then the eluent was evaporated to dryness. After that the residue was dissolved in methanol/ethyl acetate/hexane (0.3:3:10) and the solution was applied to a BOND ELUT DI0L(or SEP PAK-DIOL)column, BCM eluted with acetone was dissolved in the mobile phase and analyzed by HPLC on two C18 reversed phase columns. BCM-BZ extracted from tissues was also cleaned up with BOND ELUT DIOL, and analyzed by HPLC...
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