Abstract
The analysis of betaine by high-performance liquid chromatography presents some difficulties owing to the quaternary ammonium moiety. This problem was investigated on polar stationary phases such as silica gel and amine-bonded silica gel. A method for titration is described and examples of betaine analysis in commercial beet sugars and wine are given. Pre-treatment of samples is required prior to the quantitative determination of betaine by liquid chromatographic analysis. Under particular conditions, it would be possible to detect or to determine the amount of sugar added to wines in order to increase their ethanol content.
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