Abstract
Determination of berberine chloride in pharmaceutical preparations was investigated by capillary electrophoresis. Free zone capillary electrophoresis in phosphate buffer at pH 5.0 gave complete separation of berberine from accompanying substances within 20 min. A sample solution was introduced by hydrostatic method and the on-column detection was performed at 214 nm. The calibration curves of berberine chloride gave good linearity over the concentration range of 10100 μg ml-1. Berberine chloride in various preparations was determined with good reproducibility.
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