Abstract

A competitive enzyme-linked immunosorbent assay (ELISA) for the quantiation of benomyl (as carbendazim) and carbendazim in water was developed. This assay utilizes magnetic particles as the solid phase to attach polyclonal rabbit anti-carbendazim antibodies. The ELISA has an estimated detection limit of 0.1 ng/ml of carbendazim in water. It compares favorably with HPLC measurements in the analysis of water samples. Studies indicate that this immunoassay can also be used for the determination of carbendazim in fruit juice and soil

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