Abstract
Abstract A high-performance liquid chromatographic (HPLC) method for the determination of individual B6 vitamers was developed, and its application to dog, rat, monkey and human serum is described. Serum samples were deproteinated with 8% aqueous perchloric acid and the extracts were purified using an anion-exchange column. Analytical separation of the B6 vitamers and the internal standard (4′-deoxypyridoxine) was achieved by isocratic elution with a nitrile phase column in less than 30 minutes. The described methodology is simple and fast, and yields high percentage recoveries (> 90) of the vitamers with low inter- and intra-day variations.
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