Abstract
A sensitive method, using high-performance liquid chromatography (HPLC) with fluorescence detection, has been developed for the separation and determination of B2 vitamers in the body fluid of plankton. B2 vitamers are liberated from the protein moiety in the body fluid by non-hydrolytic extraction with trichloroacetic acid and analysed by the proposed HPLC method. The major B2 vitamer in the body fluid of plankton is riboflavin, although there is also evidence to suggest the presence of endogenous enzymes that can decompose flavin mononucleotide and flavin adenine dinucleotide.
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