Abstract
Objective To establish HPLC-DAD method for determination of 2 components in Baqia-sanyu granules. Methods The HPLC system consisted of the Fortis Cortecstm C18 column (4.6 mm × 250 mm, 5 μm), the mobile phase consisted of 0.05%KH2PO4 and acetonitrile, gradient elution flow rate was 1.0 ml/min, the column temperature was 30 ℃, and the UV detector was set at 290 nm. Results The linear response range of astilbin and engelitin were 0.002 2-0.044 μg and 0.012 4-0.248 μg(r=0.999 9). The average recovery were 97.0% and 97.8%. RSD were 1.61% and 1.65%. Conclusions The method high sensitive, accurate, repeatable and high specifid. It could be an important method to control the standard of this preparations. Key words: Chromatography, high pressure liquid; Astilbin; Engelitin; Baqia-sanyu Granule
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