Abstract
Determination of ascorbic acid (AA) and dehydroascorbic acid (DHAA) in juices was performed by high-performance liquid chromatography with electrochemical detection using l-cysteine as precolumn reluctant. This method was suitable for the determination of AA and total AA (AA + DHAA) in juices. The mild reduction of DHAA to AA with l-cysteine took ca. 15 min, and the retention time of AA was ca. 15 min. The detection limit (signal-to-noise ratio = 2) was ca. 0.15 ng. The method was selective and reproducible (relative standard deviation 2.6–3.2% for AA and 2.1–3.2% for total AA). The calibration graph for AA was linear in the range 0.1–10 ng. The recovery of AA was over 90% by the standard addition method.
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