Abstract
This paper presents two high performance liquid chromatographic (HPLC) methods used for the separation and determination of artificial sweeteners aspartame, acesulphame K, sodium saccharin, and sodium cyclamate in beverages and special nutritional products (special food intended for specific population groups). All four compounds are soluble in aqueous solutions and can easily be separated and determined by HPLC with a diode array detector (DAD). The first method involved separation of aspartame, acesulphame K, and sodium saccharin on a C18 column with an isocratic elution of phosphate buffer and acetonitrile as mobile phase. The second method was used to separate sodium cyclamate on a C18 column with methanol and water as mobile phase. Under optimum conditions, both methods showed good analytical performance, such as linearity, precision, and recovery. The methods were successfully applied for the analysis of real samples of soft drinks and special nutritional products.
Highlights
This paper presents two high performance liquid chromatographic (HPLC) methods used for the separation and determination of artificial sweeteners aspartame, acesulphame K, sodium saccharin, and sodium cyclamate in beverages and special nutritional products
The most common are aspartame, sodium cyclamate, acesulphame K, and sodium saccharin, which are marketed in about 90 countries of the world [3]
The EU and Croatian regulations have established maximum permitted concentrations of artificial sweeteners in food [8,9,10,11,12]. Their content in food, drinks, and dietary products is being determined using a variety of methods such as UV spectroscopy [1, 5], capillary electrophoresis [1, 4, 6], high performance liquid chromatography [1, 2, 5, 7], and ion chromatography [1, 3]
Summary
This paper presents two high performance liquid chromatographic (HPLC) methods used for the separation and determination of artificial sweeteners aspartame, acesulphame K, sodium saccharin, and sodium cyclamate in beverages and special nutritional products (special food intended for specific population groups). The EU and Croatian regulations have established maximum permitted concentrations of artificial sweeteners in food [8,9,10,11,12] Their content in food, drinks, and dietary products is being determined using a variety of methods such as UV spectroscopy [1, 5], capillary electrophoresis [1, 4, 6], high performance liquid chromatography [1, 2, 5, 7], and ion chromatography [1, 3].
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