Abstract
A new, simple, rapid, accurate and precise HPTLC method was developed. The detector response was linear for concentrations between 100-600 ng/spot (r =0.9931). The limits of detection and quantitation were 25 ng/spot and 75 ng/spot, respectively. The recovery study was carried out by standard addition method and was found to be 99.60±0.27. Statistical analysis proved that the method was precise, accurate and reproducible, and hence was suitable for the routine analysis of artemisinin.
Highlights
A new, simple, rapid, accurate and precise HPTLC method was developed
A gift sample of artemisinin was obtained from Saokin Co-operation Ltd., Vietnam
Toluene, vanillin and sulphuric acid were of AR grade
Summary
A new, simple, rapid, accurate and precise HPTLC method was developed. The detector response was linear for concentrations between 100-600 ng/spot (r =0.9931). An attempt was made to develop an HPTLC method, which was specific, accurate, precise and reproducible. A gift sample of artemisinin was obtained from Saokin Co-operation Ltd., Vietnam. Following chromatographic conditions were used: stationary phase, silica gel F254 HPTLC precoated plates, 200 μm layer thickness, mobile phase: toluene:ethyl acetate (10:1); chamber saturation time: 30 min; Sample application: 5 mm band; separation technique: ascending; temperature: 20±5 ̊; migration distance: 75 mm; scanning mode: absorbance; Detection wavelength: 520 nm; and source of radiation utilized: combination of D2 and tungsten lamps.
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