Abstract
Abstract A simple and sensitive high-performance liquid chromatographic method for the determination of aristolochic acid (AA) in rabbit plasma has been developed. Up to 0.1 ml of plasma containing AA was deproteinized by acetonitrile, which contained an internal standard (indomethacin). The supernatant was injected onto a COSMOSIL 5C18-AR column (5 μm) using acetonitrile-0.1 % phosphoric acid (60:40, v/v, pH 2.5–2.8) as the mobile phase. UV detection at 227 nm was followed by ultraviolet spectrum identification (among 200 and 380 nm) with a photodiode-array detector. The method was rapid, easily reproduced, selective and sensitive. It was applied to pharmacokinetic studies of AA in rabbit, after a 5 mg/kg intravenous administration. A biphasic phenomenon with a rapid distribution followed by a slower elimination phase was observed from the plasma concentration-time curve. Compartmental analyses yielded a two-compartment model.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
