Abstract
The use of F. religiosa might be beneficial in inflammatory illnesses and can be used for a variety of health conditions. In this article, we studied the identification of antioxidants using (DPPH) 2,2-Diphenyl-1-picrylhydrazylradical scavenging activity in Ficus religiosa, as F. religiosa is an important herbal plant, and every part of it has various medicinal properties such as antibacterial properties that can be used by the researchers in the development and design of various new drugs. The 2,2-Diphenyl-1-picrylhydrazyl (DPPH) is a popular, quick, easy, and affordable approach for the measurement of antioxidant properties that includes the use of the free radicals used for assessing the potential of substances to serve as hydrogen providers or free-radical scavengers (FRS). The technique of DPPH testing is associated with the elimination of DPPH, which would be a stabilized free radical. The free-radical DPPH interacts with an odd electron to yield a strong absorbance at 517 nm, i.e., a purple hue. An FRS antioxidant, for example, reacts to DPPH to form DPPHH, which has a lower absorbance than DPPH because of the lower amount of hydrogen. It is radical in comparison to the DPPH-H form, because it causes decolorization, or a yellow hue, as the number of electrons absorbed increases. Decolorization affects the lowering capacity significantly. As soon as the DPPH solutions are combined with the hydrogen atom source, the lower state of diphenylpicrylhydrazine is formed, shedding its violet color. To explain the processes behind the DPPH tests, as well as their applicability to Ficus religiosa (F. religiosa) in the manufacture of metal oxide nanoparticles, in particular MgO, and their influence on antioxidants, a specimen from the test was chosen for further study. According to our findings, F. religiosa has antioxidant qualities and may be useful in the treatment of disorders caused by free radicals.
Highlights
The Van Burden and Robinson method for tannin determination, the Folin–Ciocalteu system for estimation of overall phenolic level, the Dinitrosalicylic Acid (DNS) technique for estimating carbohydrate or reducing sugar analysis, and the Folin and Lowry technique for total protein content estimation are as follows: When free-radical DPPH interacts with an odd electron, the greatest absorption takes place at 517 nm
A free-radical scavenger antioxidant reacts to DPPH to form DPPHH, which has a lower absorbance than DPPH because of the lower amount of hydrogen
We demonstrated in this study that a free radical is applied in the 2,2-Diphenyl-1picrylhydrazyl (DPPH) technique for measuring the capacity of substances to serve as scavenging free radicals or hydrogen suppliers, which can be a quick, easy, and affordable approach for determining antioxidant properties
Summary
Nanotechnology has gained popularity over recent years, and nanoparticles (NPs) are frequently regarded as the field’s basis as they are a major source of various nanostructured processes or substances. Structural engineering, electronics, and medicine make extensive use of metal NPs, which can be widely found in a range of other sectors of science and technology. Most metal NPs are manufactured using chemical as well as physical procedures, including reduction with hydrothermal pyrolysis, and stabilization with numerous chemical compounds that, subsequently, are responsible for a wide range of biohazards as well as environmental problems due to the toxicity of the substances involved [1]. Many types of malignancies have consistently increased ROS-sensitive signaling pathways, which are involved in cell growth/proliferation, differentiation, protein synthesis, glucose metabolism, cell survival, and inflammation
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