Abstract
Biosynthesis of nanoparticles by using plant extracts is presently under development. The study has been focused on the biosynthesis of silver nanoparticles (AgNPs) using aqueous extract of Aloe vera gel as well as to determine their antioxidant potential. UV-Vis spectrophotometeric analysis showed surface plasmonic resonance (SPR) band at 440 nm, which is specific for AgNPs. The Transmission Electron Microscopy (TEM) revealed that the synthesized AgNPs were spherical in shape with an average particle size of 66.6 nm. Fourier Transform Infrared Spectroscopic (FTIR) analysis of the aqueous extract before and after the synthesis of AgNPs revealed the presence of different functional groups related to phenolic and polyphenolic compounds such as tannins and flavonoids, and other metabolites like proteins, which may be responsible for the synthesis and stabilization of AgNPs. The antioxidant potential of the synthesized AGAgNPs was determined by using 2, 2 Diphenyl-1- Picryl Hydrazyl (DPPH) radical scavenging, metal chelating and reducing power assay. Antioxidant assessment showed enhanced dose dependent antioxidant potential of the synthesized AgNPs as compared to the crude extract, which can gain attention of the pharmaceutical industry for preparation of antioxidants of natural origin as the synthetic ones are suspected to be carcinogenic. Present study also supports the advantages of green method for the nanoparticles synthesis.
Highlights
Antioxidant can be defined as the substance that prevents or inhibits the accumulation of free radicals in the human body
When the aqueous AGE was mixed with 1 mM AgNO3 solution and incubated at 750C for 40 minutes at neutral pH, color changed to yellowish brown (Fig. 2c), which indicates the formation of AgNPs, this is preliminary identification of AgNPs formation
Silver nanoparticles of similar size were prepared by flower extract of Rhododendron dauricum [28]
Summary
Antioxidant can be defined as the substance that prevents or inhibits the accumulation of free radicals in the human body. Number of reactive oxygen species (ROS) and reactive nitrogen species (RNS) are generated in human body as byproducts of various types of metabolic activities, which are referred as free radicals, forming a large group of reactive organic species. ROS and RNS include species such as hydroxyl radicals (OH-), superoxide radical anion (O2-), hydrogen peroxide (H2O2), singlet oxygen species [102] and nitric oxide (NO) respectively [1]. Elevated levels of ROS generate oxidative stress leading to various types detrimental effects, which includes enzyme activation/deactivation, lipid peroxidation of cellular membranes, DNA breakage, alterated lipid-protein interaction and eventually promoting mutations that initiate tumor progression [2,3]. If the balance between free radical generation and their eradication is maintained, the harmful effects caused by them in the body can be minimized
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