Determination of antioxidant activity and tannin content from Aristolochia longa leaves and roots using liquid-liquid fractionation method

Abstract

Aristolochia longa has been known by its potentiel to cure various diseases.This study was aimd to estimate the antioxidant activity and tannin content of A.longa leaves and roots. Liquid-liquid fractionation by using three solvants was experimented for extraction of tannin from leaves and roots.Then the fractions were oven-dried and powdered to determine the condensed tannin content and antioxidant activity by acidic vanillin, DPPH radical scavenging activity and ferric reducing antioxidant power (FRAP) methods.The qualitative analysis was releazed by high-performance liquid chromatography coupled to mass spectrometry (HPLC DAD-MS).The highest extraction yield was obtained by roots N-butanol fraction (5.15±0.47%).The maximum tannin content value was noted in roots N-butanol fraction (3.90±0.24 mg QUE/g DM) followed by leaves ethyl acetate fraction (3.03±0.01 mg QUE/g DM).The IC50 values of DPPH radical scavenging activity varied significantly from IC50=0.123±0.009mg/ml to 3.737±0.56 mg/ml. Since, the FRAP values ranged from EC50=0.066±0.002 mg/ml to 4,00±0,2 mg/ml. The highest antioxydant ability was observed in leaves and roots N-butanol fractions .Also, the identification by HPLC analysis showed that the roots contains some tannin compounds than leaves such as vescalagin, Roburin D and 1,3,4-tri- O -galloylquinic acid.Our study confirmed the use of A.longa to prevent and control the oxidative stress.