Abstract
Mango ginger (Curcuma amada) is a spice of high usage in pickles, sauce, culinary formulations and traditional/folk systems of medicine for therapeutic actions in Asian countries. After establishing scheme for isolation and characterisation of labdane diterpene dialdehyde [labda-8(17), 12-diene-15, 16-dial]-an anti-tubercular agent, we developed a new validated HPLC-PDA method for its quantification in C. amada. Chromatography was performed with reverse phase column in isocratic condition at a flow rate of 1.0mL/min using mobile phase of acidified water and acetonitrile. Specificity of determination was achieved with UV (190–400nm) and mass spectrum. Good linearity was obtained with correlation coefficients >0.99. In the analytical range the precision and accuracy (RSD%) values were ⩽2%. Present validated method allowed both the identification and determination of labda-8(17), 12-diene-15, 16-dial, in C. amada rhizome. The method was applied to screen the labdane diterpene dialdehyde in the samples of different geographic locations.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
