Determination of Aniline Derivatives in Water Samples by High Performance Liquid Chromatography Coupled with On-Line Flow Injection Preconcentration

Abstract

An on-line SPE-HPLC method using a cigarette filter as the sorbent was developed and applied to analyze the aniline derivatives in water samples. A preconcentration column packed with cigarette filters was used to replace a conventional sample loop on the injector valve of an HPLC for on-line SPE. With the consumption of a 7.5 mL sample solution, the enhancement factors ranged from 287 to 349 in comparison with direct injections of 20 μL sample solution. The detection limits (S/N = 3) for preconcentration of 7.5 mL of sample solution ranged from 0.27 to 5.46 μg/L at a sample throughput of 6 samples per hour.