Abstract
ABSTRACTA sensitive, rapid, efficient, and reliable method is reported for the determination of testosterone, 17α-methyltestosterone, trenbolone, trenbolone acetate, boldenone, nandrolone phenylpropionate, nandrolone, and norethindrone in porcine hair. This protocol involves treating 100 mg of pulverized hair by mild digestion using tris(2-carboxyethyl)phosphine hydrochloride and subsequent extraction of the anabolic hormones with methyl tert-butyl ether. The separation of the analytes was performed using acetonitrile and 0.1% aqueous formic acid. Quantitative analysis was performed by high-performance liquid chromatography–tandem triple-quadrupole mass spectrometry with positive electrospray ionization. This method is accurate, offers good sensitivity and high recovery, allowing the determination of anabolic hormones in porcine hair.
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