Abstract
To investigate the chemical structure of cell wall mannan obtained from pathogenic yeast, Candida tropicalis NBRC 1400 (former antigenic standard strain, IFO 1400). As a result of two-dimensional NMR analysis, it was shown that the mannan of this strain is composed of α-1,6-, α-1,3-, α-1,2- and β-1,2-linked mannose residues. In this research, the mannan was subjected to three degradation procedures, acid-treatment, α-mannosidase, and acetolysis under two conditions in order to determine the chemical structure of the antigenic oligomannosyl side chains in this molecule. The 1H-nuclear magnetic resonance spectra of resultant oligosaccharides, pentaose and hexaose, demonstrated the existence of the oligomannosyl side chains corresponding to Manα1-3Manα1-2Manα1-2Manα1-2Man and Manα1-3Manα1-2Manα1-2Manα1-2Manα1-2Man, respectively, which have previously also been found in Candida albicans serotype A strain mannans. These findings indicate that C. tropicalis and C. albicans serotype A have no significant difference in the chemical structure of these cell wall mannans. Therefore, it can be interpreted that it is extremely difficult to distinguish both species by targeting the antigenic group in these mannans.
Highlights
Mannan present in the yeast cell wall is mainly composed of mannose residues, which forms a complex with protein and/or phospholipid [1] [2] [3] [4]
These findings indicate that C. tropicalis and C. albicans serotype A have no significant difference in the chemical structure of these cell wall mannans
We reported the structure of cell wall mannans of several Candida species, including C. tropicalis [12], C. albicans [13] [14], C. glabrata [15], C. guilliermondii [16], and C. krusei [17]
Summary
Mannan present in the yeast cell wall is mainly composed of mannose residues, which forms a complex with protein (mannoprotein) and/or phospholipid (phospholipomannan) [1] [2] [3] [4] Since these molecules are located in the outermost layer of the cell wall, it is well-known to function as major antigenic determinants [1] [2]. In order to identify clinical isolates from patients with candidiasis, Tsuchiya and his coworkers developed ten rabbit antibodies to antigenic factors of the genus Candida (abbreviated as FAbs) [8] [9] [10] These FAbs recognize the antigenic determinants in cell wall mannan [11]. The determinants of Candida factors 1, 4, 13b, and 34 are α-linked oligomannosyl side chains, a linear homologous α-1,2-linked series [12], a 3,6-branched series [22], an internal α-1,3-linkage-containing linear series [22] [23], respectively
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