Abstract
A simplified high-performance liquid chromatographic method for the determination of amoxicillin in plasma is described. Specific and sensitive fluorescence detection was achieved by on-line post-column electrochemical oxidation, using an electrochemical detector. Owing to the high specificity of the detection system, deproteinized plasma samples could be injected directly without prior treatment. This method permits a very fast and reproducible determination of amoxicillin in plasma on a routine basis at levels, down to 50 ng/ml. The absolute detection limit is about 10 pg injected.
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