Determination of Amino Acids in Plasma Samples of Preeclampsia Patients by Liquid Chromatography Coupled to High-Resolution Mass Spectrometry

Eliane Do Nascimento,Vânia Goulart,Helvécio Menezes,Zenilda Cardeal,Rodrigo Resende

doi:10.21577/0103-5053.20190093

Eliane Do Nascimento, Vânia Goulart + Show 3 more

Open Access

https://doi.org/10.21577/0103-5053.20190093

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Abstract

This work presents the application of a simple and sensitive method developed for the simultaneous analysis of the levels of twenty two underivatizated free L-amino acids in plasma samples of preeclampsia patients by high performance liquid chromatography coupled to high-resolution mass spectrometer (LC-HRMS) using hydrophilic interaction liquid chromatography (HILIC) silica column. The linearity was evaluated in the range of 0.009 to 8.018 µmol L-1 showing coefficients of determination (R2) in the range of 0.999 to 0.911 (p < 0.05). The intra- and inter-day precisions were above of 9.84 and 15.31, respectively. The limits of detection (LOD) and quantification (LOQ) were in the range of 0.003 to 0.683 µmol L-1 and 0.009 to 2.276 µmol L-1, respectively. The results of the investigation of free L-amino acids showed that the levels of GABA, Arg, Ser, Leu, Ile, Met, Val, Ala, Gly, Bet, His and Lys in plasma of patients with preeclampsia were significantly different from healthy patients.

Highlights

The direct analyses of free amino acids (AA) is very important in metabolomics studies and have been an important implement of search for understanding, diagnostic and treatment of various diseases.[1,2,3,4,5,6,7] Amino acids are important metabolites for various metabolic pathways, contributing for several biochemical reactions that involves a wide metabolic network within living cell
Amino acids play an important role in connecting the diverse metabolic pathways since changing the level of one amino acid in response to environmental transformation and/or genetic variation originates disruptions in the levels of several other amino acids.[8]
The intra-day precision was studied analyzing on the same day three concentration levels in Determination of Amino Acids in Plasma Samples of Preeclampsia Patients

Summary

Introduction

The direct analyses of free amino acids (AA) is very important in metabolomics studies and have been an important implement of search for understanding, diagnostic and treatment of various diseases.[1,2,3,4,5,6,7] Amino acids are important metabolites for various metabolic pathways, contributing for several biochemical reactions that involves a wide metabolic network within living cell. Amino acids of high-purity (≥ 98%), L-tryptophan (Trp), L-phenylalanine (Phe), L-tyrosine (Tyr), L-leucine (Leu), L-isoleucine (Ile), L-methionine (Met), L-valine (Val), L-glutamic acid (Glu), γ-aminobutyric acid (GABA), L-aspartic acid (Asp), L-alanine (Ala), L-threonine (Thr), L-serine (Ser), L-glycine (Gly), L-proline (Pro), L-sarcosine (Sar), L-asparagine (Asn), L-glutamine (Gln), L-betaine (Bet), L-arginine (Arg), L-histidine (His), L-lysine (Lys) were purchased from Sigma-Aldrich (St. Louis, MO, USA).

Results

Conclusion