Abstract
Based on solvent-free gas chromatography of the n-propyl. N-acetyl derivatives, procedures are described for the routine determination of amino acid profiles in biological samples including protein hydrolysates, plant tissue extracts, urines and sera. Derivatization is carried out in a 25-mifn two-step procedure. Use of a basic acylation environment allows derivatization of arginine and histidine. Chromatography using capsule injection on to a polar column with temperature programming gives resolution of 21 common amino acids within 15 min. Norleucine used as the internal standard provides quantitative capability. Sensitivity of the method permits quantitation at the nanomote level. Recoveries of amino acids added to sera ranged over 90–99%; an exception was arginine which gave 78%. Typical reproducibility data indicate that a coefficient of variation of 2–5% is attainable.
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