Determination of Amino Acid Pairs Sensitive to Variants in Human Bruton's Tyrosine Kinase by means of a Random Approach

Abstract

In this data-based theoretical analysis, we use a random approach to analyse amino acid pairs in human Bruton's tyrosine kinase (BTK) in order to determine which amino acid pairs are more sensitive to 112 variants with missense mutant in human BTK. The rationale of this study is based on our hypothesis and previous findings that variance is more likely to occur at randomly unpredictable amino acid pair position rather than at randomly predictable positions. This is reasonable to argue, as randomly predictable amino acid pairs are less likely to be deliberately evolved, whereas randomly unpredictable amino acid pairs are probably deliberately evolved in connection with protein function. A 91.96% of 112 variants occurred at randomly unpredictable amino acid pairs, which account for 72.49% of amino acid pairs in BTK, and the chance of a variant occurring is 3.4-fold higher in randomly unpredictable amino acid pairs than in predictable ones. Thus randomly unpredictable amino acid pairs are more sensitive to variance in human BTK. The results also suggest that the human BTK has a natural tendency towards variants.