Abstract
Two new methods for the determination of amineptine (AMN) and amprolium (AMP) have been developed. The methods consist of extractin the ion - pairs between the drug and the inorganic complex [Co (SCN)₄]-2. The optimal experimental conditions of both methods including pH, concentration of Co (II) and tlxocyanate ions, and the organic solvents were studied. The optimum pH was found to be 3.9, nitrobenzene proved to be the most suitable solvent, giving quantitative extraction for the two drugs. The two drugs can be determined in the organic phase spectrophotometrically at 625 nm showing Sandell sensitivities of 0.19 and 0.12 µg cm-2 with relative standard deviation of 0.46 and 0.87 % for amineptine and amprolium, respectively.The indirect method was also applied to measure cobalt in the organic phase by atomic absorption spectrometry at 240.7 nm, and the relative standard deviation of the method is approximately 0.35 and 0.29 % for amineptine and amprolium, respectively. The proposed methods were found to be suitable for the accurate, simple and rapid analysis of amineptine and amprolium hydrochlorides in the bulk drugs and in pharmaceutical forms.
Highlights
Amineptine hydrochloride (AMN) 7-[(lo, 11-dihydrodibenzo [a,d] cyclohepten-5-yl)amino] heptanoic acid hydrochloride, is a tricyclic antidepressant
On mixing aqueous solutions of tetrathiocyanatocobaltate (11) AMN or AMP in acidlc medium, green ion-pairs are formed via the protonated nitrogen atom of both drugs as previously shown in literature [12].The extraction of these ion-pairs with different solvents such as chloroform, 1, 2- dichloroethane, dichloromethane, ethylacetate, o- nitrotoluene and nitrobenzene were studied
The data show that 3.5- 4.0 ml of 1 M ammonium thiocyanate is required for maximum absorbance in a final volume of 10 ml aqueous solution and in presence of 0.5 ml (2 mg/ml) and 0.5 ml for AMN and AMP, respectively, after this, the absorbance was nearly constant
Summary
Amineptine hydrochloride (AMN) 7-[(lo, 11-dihydrodibenzo [a,d] cyclohepten-5-yl)amino] heptanoic acid hydrochloride, is a tricyclic antidepressant. As the hydrochloride it is formulated in tablets. Amineptine has been determined by gas chromatographic-mass fi-agmentographic [I], HPLC [2, 31, gas chromatographic- mass spectrometric [4], spectrophotometric [5] and ion-selective electrode [6] methods. Ylmethy1)-2methylpyridinium chloride hydrochloride, is an antiprotozoal used in veterinary practice, alone or in conjunction with other drugs such as ethopabate, for the control of coccidiosis, it is formulated in oral. F.M. AbouAttia : solution for veterinary use only. Pharmacopoeial methods [7, 81 for its determination in bulk and its dosage forms involve non-aqueous titration and chromatography. One extraction- spectrophotometric method [9] was applied
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