Abstract
A simple and easy liquid chromatographic procedure was developed for the separation and analysis of α,β-arteether. The developed method uses a reversed-phase Luna C18 column (250 mm × 4.6 mm), a hybrid micellar mobile phase containing 0.15 M SDS–6% (v/v) butanol–0.01 M phosphate buffered at pH 7, and detection at 218 nm. Under these conditions, the analysis time was less than 6 min. Validation studies were performed according to the International Conference on Harmonization guideline, including linearity (R2 > 0.9998) in the 0.1–50 µg/mL range, accuracy (99.8–100.8%), repeatability [RSD (%) 1.8–2.8], intermediate precision [RSD (%) 1.8–2.9], and robustness, which were found satisfactory. Method was applied to the determination of α,β-arteether in different pharmaceutical preparations and wrapper/label free ampoules obtaining recoveries in the 97.7–102.2% range. Compared to other chromatographic techniques, micellar liquid chromatography has the advantages of avoiding sample extraction from matrices, thus reducing the global analysis time and environmental toxicity.
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