Determination of albumin in caprine serum

Abstract

Dye-binding techniques using bromocresol green (BCG) and bromocresol purple (BCP) were compared with cellulose acetate electrophoresis for the determination of caprine serum albumin. When a caprine standard was used the electrophoretic values showed little difference from those obtained with BCG. However, when a bovine standard or BCP were used, there were differences. Bias was detected between the values determined with bovine and caprine albumin standards owing to the different absorbances of the dye-protein mixtures. Using BCG, a closer correlation was obtained between the electrophoretic and caprine standard values than between the electrophoretic and bovine standard values, but with BCP the reverse was true although these differences were not significant. BCP was considered a less suitable dye than BCG for routine analysis because of low absorbance changes and the resulting poor precision.