Abstract
A high-performance liquid chromatography (HPLC) method with fluorescence detection for the determination of aflatoxins B1, B2, G1, G2, and M1 in yogurt using dispersive liquid–liquid microextraction as alternative sample treatment has been developed. To enhance the fluorescence of aflatoxins B1 and G1, a post-column photochemical derivatization has been proposed, avoiding the use of derivatization reagents. The method was validated using natural yogurt as representative matrix, showing a good linearity in the studied range (25–500 ng kg−1) and limits of quantification below the maximum level established by European Union in milk for the manufacture of milk-based products. Satisfactory recoveries ranging from 69.4 to 99.7 %, with relative standard deviations lower than 11.2 %, were obtained for all the compounds. The proposed method is simple, rapid, with low solvent consumption, inexpensive, and environmentally friendly.
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