Determination of acute toxicity indicators of AQUAdez-NUK 5

Abstract

Currently, there is a problem in satisfying livestock enterprises with cheap, highly effective disinfectants. Import substitution of the vacated market segment requires optimization of the increasing costs of livestock companies. In this regard, preclinical studies of the AKVAdez-NUK 5 disinfectant were developed and studied, in particular, indicators of acute toxicity. In the experiment, males of the Wistar breed weighing 220-230 g were used, to whom the test solution was injected intragastrically, using a pin-shaped needle, in doses of 2000, 3000, 4000 and 5500 mg/kg. Using of analytical and statistical tools in data processing allowed us to state that for the disinfectant AQUAdez-NUK 5 has followed levels for lethal doses. LD16=2864,91mg/kg; LD50=3676,22±347,37mg/kg; standard error for LD50 is 347,37 mg/kg; LD84=4585,86 mg/kg; LD99=5040.69 mg/kg. The clinical picture of acute poisoning in rats was manifested by short-term excitement, followed by depression. Tachypnea, nasal discharge, impaired coordination of movement and clonic-tonic seizures were diagnosed in laboratory animals. The rats huddled in groups. The death of animals occurred due to respiratory arrest. By analyzing the results of an acute experiment and recording the frequency of animal deaths by observation days, the average time of animal death was calculated, which was 55.92 hours. Taking into consideration of the results, we can conclude increasing in the administered dose of the drug accelerates the onset of death of a laboratory animal. Up to the level of 4000 mg / kg, only one experimental rat died on the third day after the introduction of 3000 mg / kg. With the introduction of large dosages, lethality increased sharply, reaching 100% with the introduction of 5500 mg of AQUAdez‑NUC 5 per 1 kg of weight. In accordance with the classification of AQUAdez‑NUC 5, it can be attributed to drugs of an acute type of action with a moderate ability to cumulate. According to government standard 12.1.007-76, AQUAdez-NUK 5 belongs to hazard class III (moderately hazardous compounds).