DETERMINATION OF ACUTE AND SUBACUTE TOXICITY OF THE DRUG BASED ON CHLORTETRACYCLINE HYDROCHLORIDE

Abstract

The article presents the results of determination of acute and subacute toxicity of the drug "Chloracil", which was made on the basis of chlortetracycline hydrochloride. In result of the conducted researches, it was found out, that a single intragastric administration of the drug according to SOU 85.2-37-736: 2011 belongs to the 4th class of toxicity - low-toxic substances.
 In determining the toxicity of the drug by the long-term use, it was found out, that the use of Chloracil in therapeutic and in 10 times higher than the therapeutic doses did not cause death of white rats. The animals of the experimental groups were active, ate well food , and the general condition of the animals did not differ from the condition of the animals of the control group, but there was a tendency to decrease the weights of liver, spleen, heart, lungs, thymus and kidneys on the background of an increase of body weight by 3.4 and 7.3% (p <0.05), compared to the values of the control group. Therefore, the use of the tested drug in above doses for 14 days does not significantly affect on the functional state of the internal organs of experimental animals.
 The study of the effect of the drug on the hematological parameters of blood by long-term use showed that in therapeutic and in 10-time higher therapeutic doses observed a tendency to decrease the hemoglobin concentration, amount of erythrocytes, leukocytes, hematocrit value, on the background of a slight an increase of platelet. In determining the value of erythrocyte indices, it was noted that the use of the drug "Chloracil" in animals of I and II experiments did not cause significant changes in mean corpuscular hemoglobin (MCH). Mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC) were at the level of the control group.
 When studying the effect of the drug on the biochemical parameters of blood serum in animals of I and II experimental groups, there was a tendency to decrease the level of creatinine, activity of AST, ALT and LP activity on the background of a slight an increase of total protein content.
 Analysis of the obtained data shows that the use of the drug "Chloracil" to white rats for 14 days in therapeutic and in 10-time higher therapeutic doses caused a slight decrease in the weight of internal organs, hematological and biochemical parameters of blood, which that in turn did not significantly effect on the general condition of animals.