Abstract
The present paper reports the determination of the activation energies and the optimum temperatures of starch hydrolysis by porcine pancreas α-amylase. The parameters were estimated based on the literature data on the activity curves versus temperature for starch hydrolysis by α-amylase from porcine pancreas. It was assumed that both the hydrolysis reaction process and the deactivation process of α-amylase were first-order reactions by the enzyme concentration. A mathematical model describing the effect of temperature on porcine pancreas α-amylase activity was used. The determine deactivation energies Ea were from 19.82 ± 7.22 kJ/mol to 128.80 ± 9.27 kJ/mol, the obtained optimum temperatures Topt were in the range from 311.06 ± 1.10 K to 326.52 ± 1.75 K. In turn, the values of deactivation energies Ed has been noted in the range from 123.57 ± 14.17 kJ/mol to 209.37 ± 5.17 kJ/mol. The present study is related to the starch hydrolysis by α-amylase. In the industry, the obtained results the values Ea, Ed, Topt can be used to design and optimize starch hydrolysis by α-amylase porcine pancreas. The obtained results might also find application in research on the pharmaceutical preparations used to treat pancreatic insufficiency or prognosis of pancreatic cancer.
Highlights
IntroductionThe starch molecules are glucose polymers linked together by α-1,4 and α-1,6 glucosidic bonds
Introduction and Idalina GonçalvesThe starch molecules are glucose polymers linked together by α-1,4 and α-1,6 glucosidic bonds
Based on experimental data on the change in the activity of α-amylase from the porcine pancreas [7,16,20,21,22] vs. temperature, values of deactivation energies Ed, β parameters and temperatures optimal Topt were determined from Equation (6)
Summary
The starch molecules are glucose polymers linked together by α-1,4 and α-1,6 glucosidic bonds. Starch is insoluble in water at room temperature [1,2]. These products are the source of complex carbohydrates. They are synthesized naturally in a variety of plants. Plants with a high starch content include corn, potato, rice, sorghum, wheat, cassava and rhizome and bulbil of Chinese jam. Starchy substances are a major part of the human diet for most people in the world, as well as many other animals [1,3]
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