Abstract
A method for the determination of acid-labile sulfide and zero-valence sulfur (by conversion to sulfide with dithiothreitol) in subchloroplast particles in the presence of sodium dodecyl sulfate is described. A procedure to eliminate interfering sulfide production from dithiothreitol alone is provided. After color development, methylene blue is separated from interfering colored substances by cellulose phosphate column chromatography. The method requires no special equipment and allows determination of acid-labile sulfide in the micromolar range.
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