Abstract
Values for the acid dissociation constant Ka of various acidic and basic compounds were determined from the pH-dependent capacity factor k' by reversed-phase high-performance liquid chromatography (HPLC) with octadecyl silane as the stationary phase. Both the neutral and ionic forms of acids and bases were retained on the stationary phase, and k' of the ionic form markedly influenced the calculation of Ka value. Values of Ka determined by HPLC agreed well with those determined by the conventional potentiometric method, indication the availability of this method for the determination of Ka values. The hydrophobicity of the ionized forms of these compounds is discussed in terms of the partition coefficient of the neutral form between octanol and water.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
