Determination of ABO blood grouping from dentine and pulp by absorption-elution technique

Abstract

Introduction: Blood grouping has been one of the cornerstones of identification of biological material. Mostly, teeth and bones are the only significant tissues remaining in mass disasters such as aircraft crash or, bomb blasts and hence, used in human identification. It has, also, been suggested that blood group antigens in the pulp and dentine are preserved even up to 2 years after the death of an individual. Aim: In the present study, an attempt was made to determine the ABO blood grouping from the dentine and pulp by absorption-elution (AE) technique. Materials and Methods: The study group included sixty patients requiring extraction due to periodontal or, orthodontic purposes. The extraction procedure was carried out under local anesthesia following an aseptic protocol. After extraction, the socket blood was collected for blood group determination which served as control for the study. The blood grouping was performed by AE technique using powdered dentine and dental pulp. Statistical Analysis: The statistical analysis was carried out using Statistical Package for Social Sciences version 19. The statistical analysis for comparison of teeth component with ABO blood groups with the age period and gender differentiation was done using Chi-square test. P