Determination of 7 nitrobenzene compounds in soil, glass, and cotton cloth by ultrahigh performance liquid chromatography

Abstract

An ultrahigh performance liquid chromatographic (UHPLC) method has been developed for detection of 7 nitrobenzene compounds (nitrobenzene, 2-nitrotoluene, 1, 3, 5-trinitrotoluene, 3-nitrotoluene, 3, 4-dinitrotoluene, 2, 6-dinitrotoluene, 2, 4, 6-trinitrotoluene) in soil, glass and cotton cloth. Analytes were extracted twice by acetonitrile. 200 μL of the extract solution was pipetted to a tube with 800 μL water. After filtration by a nylon membrane, the analytes were determined by UHPLC with a phenyl modified column. The linear correlation coefficients of more than 0.99 were achieved. The detection range was examined from 0.05 to 5.0 mg/L. In this method, the limit of detection (LOD) of 0.5 mg/kg was obtained. Recoveries in spiked samples were more than 87.8%, and the relative standard deviations were less than 8.5%. This method is simple, and of good recovery, allowing the detection of nitrobenzene compounds in soil, glass and cotton cloth.