Abstract
The following sequence of analytical steps was used to determine the amount of 5-methylcytosine(mol-%) in calf thymus and human lymphocyte DNA: acid hydrolysis of the DNA, derivatization (pentafluorobenzyl bromide, solid phase extraction, pivalic anhydride), internal standard addition, solid phase extraction, high-performanceliquid chromatography, and gas chromatography with electron-cupture detection. The steps were carefully optimized, leading to a recovery of 30 ± 1.0% starting with a nucleobase standard containing 1.25 ng of 5-methylcytosine. A second analysis of this sample gave a 30 ± 0.3%, demonstrating a high precision for the method. In good agreement with earlier work by others, 1.2 ± 0.10 mol-% of 5-methylcytosine was then found in a 350 ng sample of calf thymus DNA, and values of 0.9 ± 0.07 and 0.8 ± 0.04 mol-% (two runs) were found in hyman lymphocyte DNA.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
