Abstract
In the present paper, microdialysis (MD) combined with high performance liquid chromatography with electrochemical detection (HPLC–ECD) was applied for the determination of 5-hydroxyindole-3-acetic acid (5-HIAA), dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in the hypothalamus of LPS-induced hyperthermia rats. The extracellular neurotransmitters of 5-HIAA, DOPAC, and HVA could be detected increasing significantly in rat brain 150 min after LPS injection. The result showed that dynamic change of neurotransmitters in rat brain could be determined easily by MD coupled with HPLC–ECD. Furthermore, the advantage that fewer animals sacrificed occurred in the microdialysis experiment was confirmed.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
