Determination of 43 pesticide residues in intact grape berries (Vitis Vinifera L.) by using an ultrasound-assisted acetonitrile extraction method followed by LC–MS/MS

Abstract

An ultrasound-assisted acetonitrile extraction method was applied for the determination of 43 pesticide residues in intact grape berries (Vitis Vinifera L.) by liquid chromatography-tandem mass spectrometry (LC–MS/MS). The results showed that ultrasound-assisted extraction from intact grape berries exhibited better efficiency than extraction from homogenized grape berries. Matrix-matched calibration curves were applied from 0.01 to 200 μg/L and determination coefficients (R2) were greater than 0.99. The recovery values were 70.6%–119.7% with a relative standard deviation (RSD) range of 0.02%–9.92%. The limits of detection (LODs) and limits of quantification (LOQs) ranged between 0.005 μg/kg and 0.2 μg/kg, and between 0.10 μg/kg and 10 μg/kg, respectively. The method was applied in the analysis of wine grape samples from three different regions of China. A total of 10 pesticides were detected in samples during fruit ripeness.