Determination of 39 veterinary drug residues in Procambarusclarkii by ultra-high performance liquid chromatography-quadrupole-time of flight mass spectrometry

Abstract

An ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UHPLC-Q-TOF-MS) method was developed for determination of 39 veterinary drugs in Procambarusclarkii. Samples were extracted with acidified acetonitrile and purified with enhanced matrix removal-lipid (EMR-Lipid) adsorbent and graphitized multi-walled carbon nanotubes (GMWNTs). Extracts were analyzed by UHPLC-Q-TOF-MS. Target compounds were screened according to accurate mass, isotopic ratio, and retention time, and confirmed by library search. Target analytes were quantified using the chromatographic peak area of each compound. For each of the drugs of interest, calibration curves were linear in their respective ranges with correlation coefficients greater than 0.99. The limits of quantification (LOQs) were 3-15 μg/kg. The recoveries for target compounds spiked in Procambarusclarkii ranged from 62.4% to 105.8%, with relative standard deviations (RSDs) ranging from 2.5% to 13.5%. This method is simple, rapid, accurate, and suitable for simultaneous determination of 39 veterinary drugs, including sulfonamides, quinolones, and triphenylmethane, extracted from Procambarusclarkii.