Abstract
It is known that almost any disease of the central nervous system is accompanied by the development of cognitive disorders. The drugs of choice in the complex therapy in this case are nootropics. On the world market racetam group, i.e. derivatives of alpha-pyrrolidone, prevails among nootropics, and the derivatives have a wide spectrum of pharmacological activity. Currently, this group continues to expand. By the employees of Perm State Pharmaceutical Academy (PSPA), ruled by Professor V.L. Gein, a new biologically active compound, a 3-pyrrolin-2-one derivative KOH-1 was synthesized. This compound is at the preclinical research stage now. The aim of this work was the development of methods for determination of KOH-1 in urine by high performance liquid chromatography (HPLC), the study of excretion KOH-1 from the organism of laboratory animals. Materials and methods . Studies on the development of methods were carried out by using a liquid chromatograph LC-20 Prominence (Shimadzu, Japan) with a diode-array detector. The validation was carried out in accordance with the requirements for bioanalytical methods, in terms of selectivity, linearity, precision and accuracy. The study of excretion of KOH-1 was performed on white non-linear male rats weighing 300-400 g. The substance KOH-1 was administered once orally in a suspension of starch mucus at a dose of 100 mg/kg. Results and discussion . As a result of the research, the method for determining the biologically active compound KOH-1 in urine has been developed. The validation showed its suitability for pharmacokinetic studies. The data on daily excretion of KOH-1 in urine after a single oral administration to rats were obtained. Conclusion. The developed conditions for the chromatographic determination of KOH-1 in urine can be used in pharmacokinetic studies, both at the preclinical and clinical stages of the study of a potential drug. The data on excretion of KOH-1 will allow to determine the ways of excretion of the preparation, and also to select a rational dosage, to identify possible contraindications to the use.
Highlights
It is known that almost any disease of the central nervous system is accompanied by the development of cognitive disorders, which are manifested by decrease in memory, attention and mental performance
The aim of this work was the development of methods for determination of KOH-1 in urine by high performance liquid chromatography (HPLC), the study of excretion KOH-1 from the organism of laboratory animals
The aim of this work was working out methods for determination of KOH-1 in urine by high performance liquid chromatography (HPLC), the study of excretion of KOH-1 from the organism of laboratory animals
Summary
It is known that almost any disease of the central nervous system is accompanied by the development of cognitive disorders, which are manifested by decrease in memory, attention and mental performance. Cognitive disorders adversely affect the quality of life of the patient and need correction [1]. The drugs of choice in the complex therapy in this case are nootropics They are the only group of pharmacological agents with a pronounced neurometabolic effect. On the world market racetam group, i.e. derivatives of alpha-pyrrolidone, prevails among nootropics, and these derivatives have a wide spectrum of pharmacological activity. A new biologically active compound [2], a 3-pyrrolin-2-one derivative – KOH-1 (Fig. 1) was synthesized
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