Abstract
A simple method for the simultaneous detection of four β 2-agonists (salbutamol, fenoterol, clorprenaline, and clenbuterol) using ion chromatography (IC) with direct conductivity detection (CD) based on their ionization in acidic medium without chemical suppression is presented. The mixture of 1.8 mM HNO 3 and 2% (v/v) acetonitrile was used as eluent. The method could be applied to the determination of the β 2-agonists in pharmaceutical preparations. The recovery of salbutamol and clenbuterol in tablets was more than 97% ( n = 3) and the relative standard deviation ( n = 11) less than 2.8%. With the proposed method, salbutamol could also be successfully detected in human plasma. In a single chromatographic run, the four β 2-agonists can be separated and determined in less than 8 min. The linear ranges were of 7.0–1.4 × 10 3 ng/ml for salbutamol, 34–7.8 × 10 3 ng/ml for fenoterol, 8.0–1.6 × 10 3 ng/ml for clorprenaline, and 25–7.5 × 10 3 ng/ml for clenbuterol. The detection limits were 2.0 ng/ml for salbutamol, 10 ng/ml for fenoterol, 3.0 ng/ml for clorprenaline, and 10 ng/ml for clenbuterol.
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