Abstract
The first sign of puberty in girls can be observed by either clinical examination or the LH and FSH response to GnRH. However, serum estradiol levels can not be detected. The aim of the study was to develop a method for determine the early increase in estrogens and therefor we focused our work on.metabolites in the urine. 24h urine was collected from healthy girls with breast development of stage B1 or B2. The volume of urine was measured, albumin added (0.1% final concentration) in the samples for analysis. Sorbent solid extraction technology was used for extraction of the steroids. BondElut extraction cartridges C18 1211-3027 were used. The estrogen content was determined with bioMeriux Estradiol enzymatic U kit (nr 6.1483), using β-glucuronidase G7771 from sigma for hydrolyzing estrogens from glucuroconjugates. Total urinary estrogens (estradiol and estrone) varied between 0.01-0.04 μg per 24h for girls in Tanner stage B1 (n=3) and between 0.23-3.45 μg per 24h for girls in Tanner stage B2 (n=4). Determination of urinary estrogens seems to be a method to determine whether puberty has started or not. This could be used both in diagnosis and treatment of precocious puberty.
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