Determination of 23 Perfluorinated Alkyl Substances in Fishery Products by Ultra Fast Liquid Chromatography-Tandem Mass Spectrometry

Abstract

A method was proposed for the determination of 23 perfluorinated alkyl substances(PFASs) in fishery products by the QuEChERS method and ultra fast liquid chromatography-tandem mass spectrometry(UFLC-MS/MS).The sample was extracted with acidified acetonitrile,cleaned-up by dispersive solid-phase extraction using C18 and graphitized carbon blacks(GCB).The separation of 23 PFASs was performed on a Kinetex XB-C18(100 mm×2.1 mm,2.6 μm) column using gradient elution of 5 mmol/L ammonium acetate and methanol as mobile phase within 12 min.The PFASs were analyzed under the multiple reaction monitoring(MRM) mode with negative electrospay ionization.Under the optimal conditions,the isotope internal standard method was used to determine the content of 23 PFASs,and improved the quantitative accuracy.The calibration curves were linear well with correlation coefficient over 0.995.The limits of detection ranged from 0.002 to 0.02 μg/kg.The average spiked recoveries for 23 PFASs were between 75.6% and 118.2%,with relative standard derivations(RSDs) from 2.7% to 14.5%.The developed method is simple and sensitive,and was suitable for rapid analysis of large quantities of samples.