Determination of 16 Selected Trace Elements in Children Plasma from China Economical Developed Rural Areas Using High Resolution Magnetic Sector Inductively Coupled Mass Spectrometry

Xiaobing Liu,Jianhua Piao,Weidong Li,Yuan Tian,Xiaoguang Yang,Shuang-Qing Zhang,Zhenwu Huang

doi:10.1155/2014/975820

Abstract

A rapid, accurate, and high performance method of high resolution sector field inductively coupled plasma mass spectrometry (HR-ICP-MS) combined with a small-size sample (0.1 mL) preparation was established. The method was validated and applied for the determination of 16 selected plasma trace elements (Fe, Cu, Zn, Rb, B, Al, Se, Sr, V, Cr, Mn, Co, As, Mo, Cd, and Pb). The linear working ranges were over three intervals, 0-1 μg/L, 0–10 μg/L and 0–100 μg/L. Correlation coefficients (R 2) ranged from 0.9957 to 0.9999 and the limits of quantification (LOQ) ranged from 0.02 μg/L (Rb) to 1.89 μg/L (Se). The trueness (or recovery) spanned from 89.82% (Al) to 119.15% (Se) and precision expressed by the relative standard deviation (RSD %) for intra-day ranging from 1.1% (Zn) to 9.0% (Se), while ranged from 3.7% (Fe) to 12.7% (Al) for interday. A total of 440 plasma samples were collected from Chinese National Nutrition and Health Survey Project 2002 (CNNHS 2002), which represented the status of plasma trace elements for the children aged 3–12 years from China economical developed rural areas. The concentrations of 16 trace elements were summarized and compared by age groups and gender, which can be used as one of the basic components for the formulation of the baseline reference values of trace elements for the children in 2002.

Highlights

The importance of trace elements and their role in biological cycling and homeostasis has become evident in recent years [1, 2], due to deficiency of essential trace elements or excess of potentially toxic elements accumulation in human body for a long time, which are responsible for various diseases [3]
The completed method of HR-ICP-MS combined with a simple-size biological sample preparation was established and applied for the multielemental analysis in practice, which can speed up analysis and limit potential sources of contamination
The objectives of our study were to apply the optimized HR-ICP-MS combined with simple small-size sample preparation to determine 16 selected plasma trace elements (Fe, Cu, Zn, Rb, B, Al, Se, Sr, V, Cr, Mn, Co, As, Mo, Cd, and Pb) in children aged 3–12 years and to formulate the reference values of 16 selected trace elements, which could be considered as the original baseline levels for the representative children living in China economical developed rural areas in 2002

Summary

Introduction

The importance of trace elements and their role in biological cycling and homeostasis has become evident in recent years [1, 2], due to deficiency of essential trace elements or excess of potentially toxic elements accumulation in human body for a long time, which are responsible for various diseases [3]. Plasma is one of the preferred biological fluids and widely used for biomonitoring purposes in the surveys involving trace elements. It is often limited by a small-size sample, complicated matrix, simultaneous multielemental analysis, and a wide range of concentrations of analytes from trace (μg/L) to ultratrace levels (ng/L-pg/L) [5]. The completed method of HR-ICP-MS combined with a simple-size biological sample preparation was established and applied for the multielemental analysis in practice, which can speed up analysis and limit potential sources of contamination

Objectives

Results

Conclusion