Abstract
Gas chromatography/isotope ratio mass spectrometry (GC/IRMS) allows the detection of low 13C enrichment. However, this method needs to set up new metabolite derivatives suitable for GC/IRMS. In this work we describe a new derivative for plasma lactate analysis. After plasma extraction, diazomethane is used to methylate the lactate carboxyl group. The lactate methylester reacts with acetic anhydride in pyridine to give an acetylated methyl ester of lactate. The isotopic 13C enrichment of this compound is determined by GC/IRMS. A correction method for the effects of the added derivative carbon atoms on measurements of the derivatized compounds is also described. This method appears reproducible and allows the detection of 13C enrichment as low as 0.01 mol.%. A practical application for the measurement of 13C plasma lactate enrichment after ingestion of an oral glucose load (0.5 g kg−1) enriched with 0.1% D-(U-13C)glucose is shown.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
