Determination of 1-aminocyclopropane-1-carboxylic acid (ACC) in leaf tissue and xylem sap using capillary column gas chromatography and a nitrogen/phosphorus detector

Abstract

The Lizada and Yang method, commonly used for analyzing 1-aminocyclopropane-1-carboxylic acid (ACC), the immediate precursor of the plant hormone ethylene, is subject to interference and lacks internal standards. The use of combined gas chromatography-mass spectrometry (GC-MS) overcomes these shortcomings but the method is expensive and unavailable to many laboratories. We describe an alternative physico-chemical method using a capillary column gas chromatograph fitted with a standard nitrogen/phosphorus detector. After forming the N-benzoyl n-propyl derivative, measurements of ACC concentrations in extracts of leaves and in xylem sap of tomato plants using the nitrogen/phosphorus detector were within 10% of those obtained by GC-MS. Concentrations in plants grown in well-drained soil were approximately 0.16 nmol g−1 fresh weight (leaves) and 0.04–0.01 mmol m−3 (sap). Flooding the soil for 48–72 h increased these values approximately 9-fold.