Abstract
A new substrate, 3-ketobutylidene β-2-chloro-4-nitrophenylmaltopentaoside (3KB-CNPG5), was used for the determination of α-amylase (EC 3.2.1.1) in serum and urine. Under this α-amylase assay condition, 3KB-CNPG5 is resistant to glucoamylase and α-glucosidase, which are auxiliary enzymes, because the 4- and 6-positions of the non-reducing-end glucose residue are modified by the 3-ketobutylidene group. The assay using 3KB-CNPG5 for α-amylase activity is a highly sensitive method that uses 2-chloro-4-nitrophenol (CNP) as an aglycone, and is a stable method for determination of α-amylase activity in biological fluids.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
