Abstract
An enzyme immunoassay (EIA) method for β-agonists has been developed using an antiserum raised in rabbits by immunization against 3- O-salbutamol succinate coupled to bovine serum albumin. Horse radish peroxidase coupled to the same salbutamol derivative was selected as tracer. The dose of salbutamol which caused 50% binding inhibition was 16.8 pg per well and the limit of detection of the EIA directly performed on diluted urine was 0.14 ng ml −1 in urine (when the variability of blank values in samples from untreated animals was taken into account). Owing to the large cross reactivities of the antibodies with a number of β-agonists, the present assay is promising for the simultaneous determination of salbutamol, clenbuterol, mabuterol, terbutaline, cimaterol and probably also other β-agonists with a tert-butyl or isopropyl group at the extremity of the aliphatic side chain of the molecule.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
