Determination, Method Development and Validation of Six Nitrosamine Impurities in Purified water by using LC-MS/MS

Abstract

N-nitroso dimethylamine (NDMA) and some other N-nitrosamines has been detected as potent mutagenic and carcinogenic arising in purified water due to the use of potable water required for production of purified water. The dominating precursors of N-nitrosamines formation during the purified water disinfection process are secondary and tertiary amines. NDMA is a disinfection of product found by the ozonation and chlorination of purified water. A high-performance liquid chromatography tandem mass spectrometry was developed to study the appearance of N-nitrosamines in purified water. The compounds were detected by Atmospheric Pressure Chemical Ionization (APCI) or Electrospray Ionization (ESI) which are the ionization modes with multiple reaction monitoring mode. Chromatographic separation was performed on a Phenomenex kinetex F5 column having the size 250 × 4.6mm, 5µ. This study proves that NMBA, NDMA, NDEA, NEIPA, NDIPA and NDBA detected in the purified water. Finally, the developed analytical method was strongly applied to the analysis of N-nitrosamines in purified water.